Enzymes and Inhibitors
Amsbio LLC Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum/ 0.5 IU
Heparinase III (EC 4.2.2.8) from Flavobacterium heparinum 0.5 IU
Apexbio Technology LLC T7 Endonuclease I(Synonyms: T7EI, T7 Endo I, T7 Endonuclease 1, T7 Endo 1, T7 mismatch-specific endonuclease), 250U.
T7 Endonuclease I (T7EI) is a recombinant DNA endonuclease produced through expression in E coli T7EI specifically targets and cleaves diverse forms of DNA substrates containing structural anomalies including mismatches of at least two base pairs heteroduplex DNA cruciform structures Holliday junctions and branched DNA intermediates as well as nicked duplexes at reduced cleavage efficiency It hydrolyzes phosphodiester bonds located near mismatched nucleotides 5 ends This enzyme is widely employed in detecting genetic mutations induced by gene-editing systems (e g CRISPR/Cas9 TALENs) identifying single nucleotide polymorphisms (SNPs) and generating randomized DNA fragments for shotgun cloning techniques
Cayman Chemical HormoneSensitive Lipase Polyc
Immunogen: synthetic peptide from the C-terminal region of human HSL receptor • Species Reactivity: Human, mouse, rat HSL receptor • Host: rabbit • Application: WB
Aldevron Inorganic Pyrophosphatase, 350ug
350ug dispense of Inorganic Pyrophosphatase. Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate.
Aldevron SpyFi Cas9 Nuclease, 1mg
5mg dispense size of SpyFi Cas9 Nuclease. From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research-Grade through Fisher, or across all quality grades (Research Grade or GMP) directly through Aldevron.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Functional Performance
•Reduced off-target effects
•Ready to use for electroporation or transfection
•10 mg/mL formulation for ease of use
LGC GENOMICS LLC (Lucigen) Plasmid-Safe ATP-Dependent DNase, 10,000 U @ 10 U/LDigest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
LGC GENOMICS LLC (Lucigen) Baseline-ZERO DNase, 5000 UDigest large or small DNA molecules better than standard DNase I.
Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
New England Biolabs, Inc. BamHI Methyltransferase – 100 units
BamHI Methyltransferase modifies the internal cytosine residue (N4) of the sequence GGATCC. This enzyme comes from an E. coli strain that carries the cloned BamHI modification gene from Bacillus amyloliquefaciens H (ATCC 49763).
New England Biolabs, Inc. PvuI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence CG_AT^CG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. HindIII-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence A^AGCT_T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.