Enzymes and Inhibitors
New England Biolabs, Inc. BstAPI – 1000 units
A restriction endonuclease that recognizes the sequence GCAN_NNN^NTGC.
New England Biolabs, Inc. BssHII – 2500 units
A restriction endonuclease that recognizes the sequence G^CGCG_C.
New England Biolabs, Inc. Nt.CviPII – 40 units
A restriction endonuclease that recognizes the sequence ^CCD. Nt.CviPII is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate. The final product on pUC19 is an array of bands from 25 to 200 bp. CCT is cut less efficiently than CCG and CCA. Some of the CCT sites are not cleaved. (Based on NEB observations.)
New England Biolabs, Inc. XbaI – 15000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. Nt.BbvCI – 1000 units
A restriction endonuclease that recognizes the sequence CC^TCAGC.
New England Biolabs, Inc. PmeI – 2500 units
A restriction endonuclease that recognizes the sequence GTTT_^AAAC.
ABclonal Technology BbsI
5...G A A G A C (N)2 ...3 3...C T T C T G (N)6 ...5 Isoschizomers: BpiI, BstV2I
